R&D公司货号M1000B 小鼠白介素10试剂盒现货促销 5000元，售完为止！

发布时间：2016-07-15 15:21 | 点击次数：348

R&D公司货号M1000B 小鼠白介素10试剂盒现货促销 5000元，售完为止！
R&D公司货号M1000B Mouse IL-10 Quantikine ELISA Kit
IL-10,白介素10,M1000B

Assay Length

4.5 hours
Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity

5.22 pg/mL
Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity

Natural and recombinant mouse IL-10
Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-10 accurately. Results obtained using natural mouse IL-10 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision over two lots of kit components. Assays were performed by at least three technicians.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	76.3	123	815	71.4	115	809
Standard Deviation	2.56	2.85	17	5.39	7.64	55.5
CV%	3.4	2.3	2.1	7.5	6.6	6.9

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	26.8	75.5	585	26.2	80	583
Standard Deviation	2.5	6	29	1.7	6.2	31
CV%	9.3	7.9	5	6.5	7.8	5.3

Recovery

The recovery of IL-10 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	99	95-103
EDTA Plasma (n=4)	91	86-99
Heparin Plasma (n=4)	89	82-98
Serum (n=4)	89	85-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-10 in each matrix were diluted with Calibrator Diluent and then assayed.

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral);

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF;

Related Research Areas

B Cell Development and Differentiation
B Cell Markers
Conventional/Classical Dendritic Cells
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Cytokines and Receptors in Tfh Cell Differentiation and Function
gamma delta T Cells
IL-10/Interferon Family
Inflammatory Mediators
Isolation, Culture, and Identification of Natural Killer Cells
Lymphoma
Macrophage Activation Markers
Monocyte Markers
Multiple Sclerosis and Myelin Related Molecules
Myeloid-derived Suppressor Cells (MDSC)
Natural Killer T (NKT) Cells
Neuroinflammation
Regulatory T Cells (Tregs)
T Cell Cytokine Signaling
Th1 Cells
Th2 Cells
Th22 Cells
Th9 Cells

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